In Vitro Activity of 3 Commercial Bacteriophage Cocktails Against Salmonella and Shigella spp. Isolates of Human Origin

Bernasconi, Odette Joëlle; Donà, Valentina; Tinguely, Regula; Endimiani, Andrea (2018). In Vitro Activity of 3 Commercial Bacteriophage Cocktails Against Salmonella and Shigella spp. Isolates of Human Origin. Pathogens and Immunity, 3(1), pp. 72-81. Case Western Reserve University, Cleveland, OH 10.20411/pai.v3i1.234

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Background: Salmonella and Shigella spp. are two of the most frequent and deadly enteric bacterial pathogens recorded worldwide. In developing countries, Salmonella infections are responsible for many deaths annually and these mortality rates are prone to increase due to the emergence of resistance to antibiotics. In this overall scenario, new alternative therapeutic approaches are needed. Methods: For the first time, we investigated the activity of three commercial bacteriophage cocktails (INTESTI, Septaphage, PYO) against a collection of contemporary Salmonella spp. (n=30) and Shigella spp. (n=20) strains isolated in Switzerland. Phage susceptibility was determined by implementing the spot test. Results: The overall susceptibility of Salmonella spp. to INTESTI and Septaphage was 87% and 77%, respectively. With regard to Shigella spp., the overall susceptibility to INTESTI and Septaphage was 95% and 55%, respectively. PYO showed to be active against only 10% of Salmonella spp. but to 95% of Shigella spp. Conclusions: Our results seem promising, especially for the INTESTI biopreparation against Salmonella enterica infections. Nevertheless, such speculation should be supported by further in vivo studies to confirm its efficacy and to prove its safety. We also emphasize the importance of large in vitro screening analyses aimed to assess the activity of such biopreparations against contemporary multidrug-resistant strains that are emerging worldwide.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > General Bacteriology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Bernasconi, Odette Joëlle; Donà, Valentina; Tinguely, Regula and Endimiani, Andrea

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2469-2964

Publisher:

Case Western Reserve University, Cleveland, OH

Funders:

[4] Swiss National Science Foundation

Projects:

[577] SNF project 153377 to Andrea Endimiani

Language:

English

Submitter:

Andrea Endimiani

Date Deposited:

21 Aug 2018 10:59

Last Modified:

24 Oct 2019 17:20

Publisher DOI:

10.20411/pai.v3i1.234

BORIS DOI:

10.7892/boris.119344

URI:

https://boris.unibe.ch/id/eprint/119344

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