The EDTA-based disk-combination tests are unreliable for the detection of MCR-mediated colistin-resistance in Enterobacteriaceae

Clément, Mathieu; Büdel, Thomas; Bernasconi, Odette Joëlle; Principe, Luigi; Perreten, Vincent; Luzzaro, Francesco; Endimiani, Andrea (2018). The EDTA-based disk-combination tests are unreliable for the detection of MCR-mediated colistin-resistance in Enterobacteriaceae. Journal of microbiological methods, 153, pp. 31-34. Elsevier 10.1016/j.mimet.2018.08.008

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We evaluated several EDTA-based combined-disk tests to detect 25 mcr producers among 48 Enterobacteriaceae. Colistin disks plus EDTA (292/584 µg) on MH and CAMH agar were used. Results were positive if with chelator there was an inhibition zone increase ≥3mm compared to colistin alone. All tests resulted unreliable (sensitivity ≤68%).

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