The EDTA-based disk-combination tests are unreliable for the detection of MCR-mediated colistin-resistance in Enterobacteriaceae

Clément, Mathieu; Büdel, Thomas; Bernasconi, Odette Joëlle; Principe, Luigi; Perreten, Vincent; Luzzaro, Francesco; Endimiani, Andrea (2018). The EDTA-based disk-combination tests are unreliable for the detection of MCR-mediated colistin-resistance in Enterobacteriaceae. Journal of microbiological methods, 153, pp. 31-34. Elsevier 10.1016/j.mimet.2018.08.008

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We evaluated several EDTA-based combined-disk tests to detect 25 mcr producers among 48 Enterobacteriaceae. Colistin disks plus EDTA (292/584 µg) on MH and CAMH agar were used. Results were positive if with chelator there was an inhibition zone increase ≥3mm compared to colistin alone. All tests resulted unreliable (sensitivity ≤68%).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > General Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Clément, Mathieu; Büdel, Thomas; Bernasconi, Odette Joëlle; Perreten, Vincent and Endimiani, Andrea

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0167-7012

Publisher:

Elsevier

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Andrea Endimiani

Date Deposited:

23 Aug 2018 09:42

Last Modified:

24 Oct 2019 09:34

Publisher DOI:

10.1016/j.mimet.2018.08.008

BORIS DOI:

10.7892/boris.119489

URI:

https://boris.unibe.ch/id/eprint/119489

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