Peptide Ligands of AmiA, AliA, and AliB Proteins Determine Pneumococcal Phenotype

Nasher, Fauzy Negiub Ali; Aguilar, Fernando; Aebi, Susanne; Hermans, Peter WM; Heller, Manfred; Hathaway, Lucy Jane (2018). Peptide Ligands of AmiA, AliA, and AliB Proteins Determine Pneumococcal Phenotype. Frontiers in Microbiology, 9(3013) Frontiers 10.3389/fmicb.2018.03013

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The Ami-AliA/AliB oligopeptide permease of Streptococcus pneumoniae has been suggested to play a role in environmental sensing and colonisation of the nasopharynx by this human bacterial pathogen by binding peptides derived from bacterial neighbours of other species in the microbiota. Here, we investigated the effects of the peptide ligands of the permease’s substrate binding proteins AmiA, AliA, and AliB on pneumococcal phenotype. AmiA and AliA ligands reduced pneumococcal growth, increased biofilm production and reduced capsule size. In contrast, AliB ligand increased growth and greatly increased bacterial chain length. A decrease in transformation rate was observed in response to all three peptides. Changes in protein expression were also observed, particularly those associated with metabolism and cell wall synthesis. Understanding interspecies bacterial communication and its effect on development of colonising versus invasive phenotypes has the potential to reveal new targets to tackle and prevent pneumococcal infections.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Nasher, Fauzy Negiub Ali; Aebi, Susanne; Heller, Manfred and Hathaway, Lucy Jane

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1664-302X

Publisher:

Frontiers

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

28 Dec 2018 11:18

Last Modified:

28 Dec 2018 11:27

Publisher DOI:

10.3389/fmicb.2018.03013

Uncontrolled Keywords:

Streptococcus pneumoniae interspecies communication growth biofilm capsule chaining ABC transporters

BORIS DOI:

10.7892/boris.122256

URI:

https://boris.unibe.ch/id/eprint/122256

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