Dommann, Noëlle; Sánchez Taltavull, Daniel; Eggs, Linda; Birrer, Fabienne Esther; Brodie, Tess; Salm, Lilian; Baier, Felix Alexander; Medova, Michaela; Humbert, Magali; Tschan, Mario; Beldi, Guido; Candinas, Daniel; Stroka, Deborah
(2020).
The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer.
Cancers, 12(7)
MDPI AG
10.3390/cancers12071913
Colorectal cancer, along with its high potential for recurrence and metastasis, is a major health burden. Uncovering proteins and pathways required for tumor cell growth is necessary for the development of novel targeted therapies. Ajuba is a member of the LIM domain family of proteins whose expression is positively associated with numerous cancers. Our data shows that Ajuba is highly expressed in human colon cancer tissue and cell lines. Publicly available data from The Cancer Genome Atlas shows a negative correlation between survival and Ajuba expression in patients with colon cancer. To investigate its function, we transduced SW480 human colon cancer cells, with lentiviral constructs to knockdown or overexpress Ajuba protein. The transcriptome of the modified cell lines was analyzed by RNA sequencing. Among the pathways enriched in the differentially expressed genes, were cell proliferation, migration and differentiation. We confirmed our sequencing data with biological assays; cells depleted of Ajuba were less proliferative, more sensitive to irradiation, migrated less and were less efficient in colony formation. In addition, loss of Ajuba expression decreased the tumor burden in a murine model of colorectal metastasis to the liver. Taken together, our data supports that Ajuba promotes colon cancer growth, migration and metastasis and therefore is a potential candidate for targeted therapy.
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Journal Article
(Original Article)
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Radio-Onkologie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Radio-Onkologie 04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) 04 Faculty of Medicine > Service Sector > Institute of Pathology 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine 04 Faculty of Medicine > Service Sector > Institute of Pathology > Tumour Pathology 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
UniBE Contributor: |
Dommann, Noëlle Annick, Sánchez Taltavull, Daniel, Birrer, Fabienne Esther, Brodie, Tess Melinda, Salm, Lilian, Baier, Felix Alexander, Medova, Michaela, Humbert, Magali, Tschan, Mario Paul, Beldi, Guido Jakob Friedrich, Candinas, Daniel, Stroka, Deborah |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
2072-6694 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Beatrice Scheidegger
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Date Deposited: |
26 Aug 2020 11:14 |
Last Modified: |
05 Dec 2022 15:40 |
Publisher DOI: |
10.3390/cancers12071913 |
PubMed ID: |
32679899 |
Uncontrolled Keywords: |
Ajuba cell differentiation metastasis proliferation |
BORIS DOI: |
10.7892/boris.146067 |
URI: |
https://boris.unibe.ch/id/eprint/146067 |
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