The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer.

Dommann, Noëlle; Sánchez Taltavull, Daniel; Eggs, Linda; Birrer, Fabienne Esther; Brodie, Tess; Salm, Lilian; Baier, Felix Alexander; Medova, Michaela; Humbert, Magali; Tschan, Mario; Beldi, Guido; Candinas, Daniel; Stroka, Deborah (2020). The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer. Cancers, 12(7) MDPI AG 10.3390/cancers12071913

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Colorectal cancer, along with its high potential for recurrence and metastasis, is a major health burden. Uncovering proteins and pathways required for tumor cell growth is necessary for the development of novel targeted therapies. Ajuba is a member of the LIM domain family of proteins whose expression is positively associated with numerous cancers. Our data shows that Ajuba is highly expressed in human colon cancer tissue and cell lines. Publicly available data from The Cancer Genome Atlas shows a negative correlation between survival and Ajuba expression in patients with colon cancer. To investigate its function, we transduced SW480 human colon cancer cells, with lentiviral constructs to knockdown or overexpress Ajuba protein. The transcriptome of the modified cell lines was analyzed by RNA sequencing. Among the pathways enriched in the differentially expressed genes, were cell proliferation, migration and differentiation. We confirmed our sequencing data with biological assays; cells depleted of Ajuba were less proliferative, more sensitive to irradiation, migrated less and were less efficient in colony formation. In addition, loss of Ajuba expression decreased the tumor burden in a murine model of colorectal metastasis to the liver. Taken together, our data supports that Ajuba promotes colon cancer growth, migration and metastasis and therefore is a potential candidate for targeted therapy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Radio-Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Radio-Onkologie

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
04 Faculty of Medicine > Service Sector > Institute of Pathology > Tumour Pathology

UniBE Contributor:

Dommann, Noëlle Annick; Sánchez Taltavull, Daniel; Birrer, Fabienne Esther; Brodie, Tess Melinda; Salm, Lilian; Baier, Felix Alexander; Medova, Michaela; Humbert, Magali; Tschan, Mario; Beldi, Guido; Candinas, Daniel and Keogh-Stroka, Deborah M.

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2072-6694

Publisher:

MDPI AG

Language:

English

Submitter:

Beatrice Scheidegger

Date Deposited:

26 Aug 2020 11:14

Last Modified:

27 Apr 2021 12:16

Publisher DOI:

10.3390/cancers12071913

PubMed ID:

32679899

Uncontrolled Keywords:

Ajuba cell differentiation metastasis proliferation

BORIS DOI:

10.7892/boris.146067

URI:

https://boris.unibe.ch/id/eprint/146067

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