A lung-on-chip array with an integrated bio-inspired respiration mechanism

Stucki, Andreas; Stucki, Janick Daniel; Hall, Sean; Felder, Marcel Christian; Mermoud, Yves; Schmid, Ralph; Geiser, Thomas; Guenat, Olivier Thierry (2015). A lung-on-chip array with an integrated bio-inspired respiration mechanism. Lab on a chip, 15(5), pp. 1302-1310. Royal Society of Chemistry 10.1039/C4LC01252F

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We report about a lung-on-chip array that mimics the pulmonary parenchymal environment, including the thin, alveolar barrier and the three-dimensional cyclic strain induced by the breathing movements. A micro-diaphragm used to stretch the alveolar barrier is inspired by the in-vivo diaphragm, the main muscle responsible for inspiration. The design of this device aims not only at best reproducing the in-vivo conditions found in the lung parenchyma, but also at making its handling easy and robust. An innovative concept, based on the reversible bonding of the device, is presented that enables to accurately control the concentration of cells cultured on the membrane by easily accessing both sides of the membranes. The functionality of the alveolar barrier could be restored by co-culturing epithelial and endothelial cells that formed tight monolayers on each side of a thin, porous and stretchable membrane. We showed that cyclic stretch significantly affects the permeability properties of epithelial cell layers. Furthermore, we could also demonstrate that the strain influences the metabolic activity and the cytokine secretion of primary human pulmonary alveolar epithelial cells obtained from patients. These results demonstrate the potential of this device and confirm the importance of the mechanical strain induced by the breathing in pulmonary research.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Stucki, Andreas; Stucki, Janick Daniel; Hall, Sean; Felder, Marcel Christian; Mermoud, Yves; Schmid, Ralph; Geiser, Thomas and Guenat, Olivier Thierry

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

23 Dec 2014 13:13

Last Modified:

15 Feb 2019 09:08

Publisher DOI:

10.1039/C4LC01252F

PubMed ID:

25521475

BORIS DOI:

10.7892/boris.61288

URI:

https://boris.unibe.ch/id/eprint/61288

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