Polysaccharide capsule composition of pneumococcal serotype 19A subtypes: Unaltered among subtypes and independent of the nutritional environment

Brugger, Silvio; Troxler, Lukas; Rüfenacht, Susanne; Frey, Pascal Marcel; Morand, Brigitte; Geyer, Rudolf; Mühlemann, Kathrin; Höck, Stefan; Thormann, Wolfgang; Furrer, Julien; Christen, Stephan; Hilty, Markus (2016). Polysaccharide capsule composition of pneumococcal serotype 19A subtypes: Unaltered among subtypes and independent of the nutritional environment. Infection and immunity, 84(11), pp. 3152-3160. American Society for Microbiology 10.1128/IAI.00474-16

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Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the seven-valent pneumococcal conjugate vaccine (PCV7) and serotype 19A has now been included in the recent thirteen-valent vaccine (PCV13). Genetic analysis has revealed at least three different capsular serotype 19A subtypes and nutritional environment dependent variation of the 19A capsule structure has been reported. Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules. We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition in different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography – mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). After the introduction of PCV7 a significant relative increase of subtype 19A-II and decrease of 19A-I occurred. Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media being consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine and glucose. In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists. The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule variant. However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage such as antibiotic resistance or loss or acquisition of other virulence factors

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology
08 Faculty of Science > Departement of Chemistry and Biochemistry
04 Faculty of Medicine > Other Institutions > Teaching Staff, Faculty of Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Clinic of General Internal Medicine

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Brugger, Silvio; Troxler, Lukas; Rüfenacht, Susanne; Frey, Pascal Marcel; Morand, Brigitte; Mühlemann, Kathrin; Thormann, Wolfgang; Furrer, Julien; Christen, Stephan and Hilty, Markus

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
500 Science > 540 Chemistry

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Julien Henri Lucien Furrer

Date Deposited:

07 Sep 2016 11:06

Last Modified:

23 Feb 2017 02:30

Publisher DOI:

10.1128/IAI.00474-16

PubMed ID:

27550933

Additional Information:

Brugger and Troxler contributed equally to this work. Co-first authorship.

BORIS DOI:

10.7892/boris.87434

URI:

https://boris.unibe.ch/id/eprint/87434

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