Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot.

Humbert, Magali; Morán, María; de la Cruz-Ojeda, Patricia; Muntané, Jordi; Wiedmer, Tabea; Apostolova, Nadezda; McKenna, Sharon L; Velasco, Guillermo; Balduini, Walter; Eckhart, Leopold; Janji, Bassam; Sampaio-Marques, Belém; Ludovico, Paula; Žerovnik, Eva; Langer, Rupert; Perren, Aurel; Engedal, Nikolai; Tschan, Mario (2020). Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot. Biology, 9(3), pp. 1-24. MDPI 10.3390/biology9030059

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Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Tumour Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Humbert, Magali; Langer, Rupert and Tschan, Mario

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2079-7737

Publisher:

MDPI

Language:

English

Submitter:

Mario Tschan

Date Deposited:

07 May 2020 09:53

Last Modified:

07 May 2020 09:53

Publisher DOI:

10.3390/biology9030059

PubMed ID:

32245178

Uncontrolled Keywords:

autophagy biomarkers disease pathology

BORIS DOI:

10.7892/boris.143700

URI:

https://boris.unibe.ch/id/eprint/143700

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