Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

Strässle, Marina; Laloli, Laura; Gultom, Mitra; V'kovski, Philip; Stoffel, Michael H.; Crespo Pomar, Silvia; Chanfon Bätzner, Astrid; Ebert, Nadine; Labroussaa, Fabien; Dijkman, Ronald; Jores, Joerg; Thiel, Volker (2021). Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria. Veterinary microbiology, 257, p. 109067. Elsevier 10.1016/j.vetmic.2021.109067

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Respiratory diseases negatively impact the global goat industry, but are understudied. There is a shortage of established and biological relevant in vitro or ex vivo assays to study caprine respiratory infections. Here, we describe the establishment of an in vitro system based on well-differentiated caprine airway epithelial cell (AEC) cultures grown under air liquid interface conditions as an experimental platform to study caprine respiratory pathogens. The functional differentiation of the AEC cultures was monitored and confirmed by light and immunofluorescence microscopy, scanning electron microscopy and examination of histological sections. We validated the functionality of the platform by studying Influenza D Virus (IDV) infection and Mycoplasma mycoides subsp. capri (Mmc) colonization over 5 days, including monitoring of infectious agents by titration and qPCR as well as colour changing units, respectively. The inoculation of caprine AEC cultures with IDV showed that efficient viral replication takes place, and revealed that IDV has a marked cell tropism for ciliated cells. Furthermore, AEC cultures were successfully infected with Mmc using a multiplicity of infection of 0.1 and colonization was monitored over several days. Altogether, these results demonstrate that our newly-established caprine AEC cultures can be used to investigate host-pathogen interactions of caprine respiratory pathogens.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Strässle, Marina; Laloli, Laura; Gultom, Mitra Lovelin; V'kovski, Philip; Stoffel, Michael Hubert; Crespo Pomar, Silvia; Chanfon Bätzner, Astrid; Ebert, Nadine; Labroussaa, Fabien; Dijkman, Ronald; Jores, Jörg and Thiel, Volker Earl

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

13 Jul 2021 10:03

Last Modified:

18 Jul 2021 03:05

Publisher DOI:

10.1016/j.vetmic.2021.109067

PubMed ID:

33862331

Uncontrolled Keywords:

AECs ALI Air liquid interface Airway epithelial cell culture Goat Influenza D virus Mycoplasma mycoides subsp. capri

BORIS DOI:

10.48350/156607

URI:

https://boris.unibe.ch/id/eprint/156607

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