Repatriation of a patient with COVID-19 contributed to the importation of an emerging carbapenemase producer

Moser, Aline I.; Campos-Madueno, Edgar I.; Sendi, Parham; Perreten, Vincent; Keller, Peter M.; Ramette, Alban; Endimiani, Andrea (2021). Repatriation of a patient with COVID-19 contributed to the importation of an emerging carbapenemase producer. Journal of global antimicrobial resistance, 27, pp. 267-272. Elsevier 10.1016/j.jgar.2021.10.012

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BACKGROUND

Patients hospitalized abroad can become colonized with multidrug-resistant bacteria and import them to their home countries. In this work, we characterized an OXA-484 carbapenemase-producing Escherichia coli strain from a Swiss patient infected by SARS-CoV-2 and repatriated from India.

METHODS

At admission to Switzerland (April, 2021), the patient undertook a molecular nasopharyngeal swab to search for SARS-CoV-2 and a rectal swab to detect multidrug-resistant bacteria. Both SARS-CoV-2 and E. coli isolates were whole-genome sequenced and analyzed for phylogenetic relatedness.

RESULTS

The patient was infected with the SARS-CoV-2 B.1.617.2 lineage (VOC Delta), a lineage that started to be reported across Switzerland at that time. He was also colonized with a sequence type (ST) 410 E. coli strain (L3452210II) producing the OXA-484, a single amino acid variant of OXA-181. The blaOXA-484 was carried by a 51.5 kb IncX3 plasmid identical to those described in blaOXA-181-harboring ST410 E. coli strains. Core-genome analysis showed that L3452210II was identical (∆SNV ≤23) to two ST410 OXA-484 producers recently reported in Qatar and Germany, but differed from other ST410 OXA-181 producers reported worldwide.

CONCLUSIONS

The patient was infected by an emerging SARS-CoV-2 variant, and also imported an E.coli producing OXA-484, an OXA-48-like carbapenemase not yet reported in Switzerland. The genetic background of L3452210II indicated that the blaOXA-484 shared the same plasmid of blaOXA-181, but its bacterial host differed from most of the pandemic OXA-181-producing ST410 strains previously reported. This case description underlines that the COVID-19 crisis can contribute to the worldwide spread of emerging carbapenemase producers.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Clinical Microbiology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > General Bacteriology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Mycobacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Moser, Aline; Campos-Madueno, Edgar Igor; Sendi, Parham; Perreten, Vincent; Keller, Peter Michael; Ramette, Alban Nicolas and Endimiani, Andrea

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

2213-7165

Publisher:

Elsevier

Funders:

[4] Swiss National Science Foundation

Projects:

[1501] Multidrug-Resistant Enterobacterales Colonizing Swiss Embassy Employees and Relatives Worldwide: Molecular Features, Metagenomics, and Transmission to the Householders at Return Official URL

Language:

English

Submitter:

Andrea Endimiani

Date Deposited:

16 Nov 2021 17:37

Last Modified:

17 Mar 2022 10:49

Publisher DOI:

10.1016/j.jgar.2021.10.012

PubMed ID:

34718203

Uncontrolled Keywords:

IncX3 OXA-48 OXA-484 SARS-CoV-2 carbapenemases plasmid

BORIS DOI:

10.48350/160541

URI:

https://boris.unibe.ch/id/eprint/160541

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