Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus.

Gultom, Mitra; Kratzel, Annika; Portmann, Jasmine; Stalder, Hanspeter; Chanfon Bätzner, Astrid; Gantenbein, Hans; Gurtner, Corinne; Ebert, Nadine; Gad, Hans Henrik; Hartmann, Rune; Posthaus, Horst; Zanolari, Patrik; Pfaender, Stephanie; Thiel, Volker Earl; Dijkman, Ronald (2022). Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus. Scientific reports, 12(1), p. 10340. Springer Nature 10.1038/s41598-022-13777-y

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In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Gultom, Mitra Lovelin, Kratzel, Annika, Portmann, Jasmine, Stalder, Hanspeter, Chanfon Bätzner, Astrid, Gantenbein, Hans, Gurtner, Corinne, Ebert, Nadine, Posthaus, Horst, Zanolari, Patrik, Thiel, Volker Earl, Dijkman, Ronald

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

22 Jun 2022 09:57

Last Modified:

05 Dec 2022 16:21

Publisher DOI:

10.1038/s41598-022-13777-y

PubMed ID:

35725865

BORIS DOI:

10.48350/170803

URI:

https://boris.unibe.ch/id/eprint/170803

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