A new OCH β-lactamase from a Brucella pseudintermedia (Ochrobactrum intermedium) strain isolated from Zophobas morio larvae.

Kundlacz, Cindy; Aldeia, Claudia; Eddoubaji, Yasmine; Campos-Madueno, Edgar I; Endimiani, Andrea (2024). A new OCH β-lactamase from a Brucella pseudintermedia (Ochrobactrum intermedium) strain isolated from Zophobas morio larvae. Journal of global antimicrobial resistance, 36, pp. 65-69. Elsevier 10.1016/j.jgar.2023.12.012

[img]
Preview
Text
1-s2.0-S2213716523002229-main.pdf - Accepted Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (1MB) | Preview
[img]
Preview
Text
Final-1-s2.0-S2213716523002229-main.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

OBJECTIVES

OCH class C β-lactamases have been reported in several species belonging to the Brucella genus that were formerly known as Ochrobactrum. Moreover, only one complete genome of Brucella pseudintermedia has been published. In this work, we describe the genome of a B. pseudintermedia strain possessing a new blaOCH gene that was isolated from Zophobas morio larvae.

METHODS

Hybrid whole-genome sequencing analysis (Illumina and Nanopore) was used to identify and characterize the strain (Ops-OCH-23). Phylogenetic analyses based on the 16S rRNA gene sequence and a core-genome alignment were performed to study the relationships among Ops-OCH-23 and deposited genomes. Moreover, all deposited blaOCH genes were compared to the one found in Ops-OCH-23.

RESULTS

Ops-OCH-23 showed a susceptibility profile consistent with the production of AmpC β-lactamase(s). Its genome consisted of two chromosomes, of which one carried the blaOCH gene. Such gene encoded a new class C OCH β-lactamase among the fifteen so far reported. Two plasmids (120-Kb and 59-Kb) without any associated antimicrobial resistance genes were also found. Analysis of 16S rRNA revealed that Ops-OCH-23 shared 100% homology with four deposited B. pseudintermedia strains. Moreover, core-genome analysis indicated that the closest match (279 ΔSNVs) to Ops-OCH-23 was strain CTOTU49018 isolated from an urban environment in Germany in 2013.

CONCLUSION

We described the second complete genome of a B. pseudintermedia that also encoded a new OCH β-lactamase variant. Overall, this report expands our knowledge regarding this rarely isolated Brucella species that have been reported so far only few times in human sources.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kundlacz, Cindy, Azevedo da Aldeia, Claudia Cristina, Eddoubaji, Yasmine, Campos-Madueno, Edgar Igor, Endimiani, Andrea

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2213-7165

Publisher:

Elsevier

Funders:

[4] Swiss National Science Foundation

Projects:

Projects 206400 not found.

Language:

English

Submitter:

Pubmed Import

Date Deposited:

28 Dec 2023 12:22

Last Modified:

10 May 2024 07:30

Publisher DOI:

10.1016/j.jgar.2023.12.012

PubMed ID:

38128729

Uncontrolled Keywords:

AmpC Brucella pseudintermedia Ochrobactrum Zophobas morio bla(OCH)

BORIS DOI:

10.48350/190671

URI:

https://boris.unibe.ch/id/eprint/190671

Actions (login required)

Edit item Edit item
Provide Feedback