Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2

Gultom, Mitra; Licheri, Matthias; Laloli, Laura; Wider, Manon; Strässle, Marina; V'kovski, Philip; Steiner, Silvio; Kratzel, Annika; Tran, Thi Nhu Thao; Probst, Lukas; Stalder, Hanspeter; Portmann, Jasmine; Holwerda, Melle; Ebert, Nadine; Stokar-Regenscheit, Nadine; Gurtner, Corinne; Zanolari, Patrik; Posthaus, Horst; Schuller, Simone; Vicente-Santos, Amanda; ... (2021). Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2. Emerging infectious diseases, 27(7), pp. 1811-1820. Centers for Disease Control and Prevention 10.3201/eid2707.204660

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Severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) has spread globally, and the number
of worldwide cases continues to rise. The zoonotic origins
of SARS-CoV-2 and its intermediate and potential
spillback host reservoirs, besides humans, remain
largely unknown. Because of ethical and experimental
constraints and more important, to reduce and refi ne
animal experimentation, we used our repository of welldiff
erentiated airway epithelial cell (AEC) cultures from
various domesticated and wildlife animal species to
assess their susceptibility to SARS-CoV-2. We observed
that SARS-CoV-2 replicated effi ciently only in monkey
and cat AEC culture models. Whole-genome sequencing
of progeny viruses revealed no obvious signs of
nucleotide transitions required for SARS-CoV-2 to productively
infect monkey and cat AEC cultures. Our fi ndings,
together with previous reports of human-to-animal
spillover events, warrant close surveillance to determine
the potential role of cats, monkeys, and closely related
species as spillback reservoirs for SARS-CoV-2.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Gultom, Mitra Lovelin; Licheri, Matthias; Laloli, Laura; Wider, Manon Flore; Strässle, Marina; V'kovski, Philip; Steiner, Silvio; Kratzel, Annika; Tran, Thi Nhu Thao; Probst, Lukas Martin; Stalder, Hanspeter; Portmann, Jasmine; Holwerda, Melle; Ebert, Nadine; Gurtner, Corinne; Zanolari, Patrik; Posthaus, Horst; Ruggli, Nicolas; Thiel, Volker Earl and Dijkman, Ronald

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1080-6059

Publisher:

Centers for Disease Control and Prevention

Language:

English

Submitter:

Nathalie Viviane Zollinger

Date Deposited:

25 Jun 2021 11:53

Last Modified:

25 Jun 2021 11:53

Publisher DOI:

10.3201/eid2707.204660

PubMed ID:

34152956

BORIS DOI:

10.48350/157091

URI:

https://boris.unibe.ch/id/eprint/157091

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